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Comparison of mutant and parent lifecycles. The attenuated mutant (A) develops from arthroconidia into non-rupturing spherules that do not contain endospores. (B) In contrast, the wild-type strain begins to develop endospores around 96 h. These structures rupture near 120 h, releasing endospores which individually develop into mature, rupturing spherules every 120 h. Illustration created with BioRender.
