KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
S. cerevisiae α-factor	Bio-Synthesis	WHWLQLKPGQPMY (custom peptide synthesis)
G418	Thermo Fisher	Cat#11811031
Cycloheximide	Millipore Sigma	Cat#C7698
Deposited Data
Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters, Srikant S et al.	Mendeley Dataset	https://doi.org/10.17632/rp3hnvh494.1
Experimental Models: Organisms/Strains
Yeast strains	See Table S2	N/A
Oligonucleotides
Primers	See Table S4	Custom oligo synthesis
Software and Algorithms
SWISS-MODEL	[58, 59]	https://swissmodel.expasy.org/
PyMol Molecular Graphics System	[60]	https://pymol.org/2/
Consurf	[61, 62]	https://consurf.tau.ac.il/
Fiji	[63, 64]	https://imagej.net/Fiji
Analysis scripts	https://github.com/sriramsrikant/2020-CB_ABC-selectivity	Python scripts
HMMER	[65]	http://hmmer.org/
Other
Atomizer	Oenophilia	Cat#900432