Fig. 5.

AR directly binds to the promoter of miR-146a-5p to suppress its transcription. A) Illustration for wild type (WT) and mutant promoter regions of miR-146a-5p. B) In HA22T cells, DNA fragment binding with AR was pulled-down using ChIP assay. Semi-quantitative PCR (left) and qPCR (right) analyses using primers that flanked the AR DNA binding site (ARE1 or ARE2) in the promoter region of the miR-146a-5p. C) PLKO or shAR SK-HEP-1 cells were transiently co-transfected with luciferase reporter plasmid (pGL3 WT or pGL3 mutant) and pRL-TK vector (control) for 2 days. Luciferase activities were presented in ratios of Firefly to Renilla luciferase reporter activities. Data are presented as mean ± SD. *P < 0.05, NS=Not Significant.