FIGURE 1.

Analysis of unreduced male gametes in Cymbidium cultivars using the squash method. (A) Mature pollens were stained with carbolfuchsin, and dyad (a), triad (b), and tetrad (c) gametes were identified by the squash method. Bar = 10 μm. The dyad with 2n gametes (arrow) (d), triads with one unreduced gamete (arrow) and two reduced gametes (e), and tetrad with four reduced gametes (f) were stained with 4’,6-diamidino-2-phenylindole (DAPI). Bar = 50 μm. (B) 2n gamete formation frequencies (means of 3-year data, Supplementary Table S1) among nine cultivars, bars represent standard error (n = 30). Different letters on the top of bars indicate significant cultivar difference in 2n gamete formation frequencies analyzed by Duncan’s multiple range test at P < 0.05 level.