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. Author manuscript; available in PMC: 2020 May 22.
Published in final edited form as: Nat Protoc. 2019 Oct 18;14(11):3033–3058. doi: 10.1038/s41596-019-0203-1

Figure 4 |.

Figure 4 |

End point analyses of mouse and human differentiations. (a) Flow cytometry analysis of CHX10 at D6 of mouse differentiation represented in a scatter plot and histogram. (b) Flow cytometry analysis of CHX10 at D17 of human differentiation represented in a scatter plot and histogram. (c) Immunocytochemistry for CHX10 (green) and Hoechst (blue) on plated mouse aggregates at D6. Scale bar = 100 μm, (d) Immunocytochemistry for CHX10 (green) and Hoeschst (blue) on human monolayer cultures at D17. Scale bar = 100 μm, (e) qPCR on D6 mouse cultures. Reported fold change is compared to cultures that did not receive DAPT, n = 3 from distinct samples. (f) qPCR on D17 human cultures. Reported fold change is compared to PSCs. Error bars indicate standard deviation, n = 3 from distinct samples.