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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Acta Neuropathol. 2019 Oct 29;139(1):157–174. doi: 10.1007/s00401-019-02086-w

Fig 4. Variable DNA damage repair in neurofibroma cells from patients with unknown ATM status.

Fig 4.

(a) Analysis of γH2AX foci in nuclei of peripheral nerve and neurofibroma tissue sections. The percent of nuclei with 0, 1–5, or >5 γH2AX foci within a nucleus was calculated. (b) 53BP1 foci in Schwann cells cultured from DNFs. The percent of nuclei with 0–3, 4–7 or >7 53BP1 foci was counted 1 recovery after exposure to 0.1uM Doxorubicin (2-way ANOVA). (c) Comet assay. Time course shows the percent of nuclei with DNA comet tail moment >50 in each of 4 individual DNF samples exposed to 1uM doxorubicin. Repair was delayed in 2 of the samples. Images of DNA comet with DNA damage repaired (upper right) and unrepaired (lower right).