Table 2.
Ct values for nCoV-QS and 2019-nCoV CDC EUA RT-qPCR using CDC adapted protocol for 25 samples (a) and MiCoBioMed protocol for 29 samples (b).
| Sample | Conc. [copies/μL] | CT (2019-nCoV CDC EUA) |
Result (2019-nCoV CDC EUA) | CT (nCoV-QS) |
Result (nCoV-QS) | |||
|---|---|---|---|---|---|---|---|---|
| N1 | N2 | RP | ORF3a | N | ||||
| 1 | 193453.3 | 23.02 | 24.8 | 25.04 | Positive | 28.72 | 24.48 | Positive |
| 2 | 151418.7 | 23.34 | 25.16 | 22.69 | Positive | 32.19 | 24.63 | Positive |
| 3 | 28204.0 | 25.53 | 28.02 | 26.11 | Positive | 30.78 | 27.3 | Positive |
| 4 | 7157.3 | 27.31 | 29.82 | 25.43 | Positive | 34.76 | 30.31 | Positive |
| 5 | 699.3 | 30.34 | 33.23 | 25.32 | Positive | 37.29 | 32.25 | Positive |
| 6 | 684.1 | 30.37 | 33.3 | 25.29 | Positive | N/A | 33.58 | Positive |
| 7 | 657.4 | 30.42 | 32.37 | 29.23 | Positive | 38.26 | 32.16 | Positive |
| 8 | 261.6 | 31.62 | 33.76 | 27.32 | Positive | 39.88 | 33.67 | Positive |
| 9 | 236.5 | 31.75 | 34.4 | 21.73 | Positive | 39.98 | 34.24 | Positive |
| 10 | 162.4 | 32.24 | 34.87 | 23.17 | Positive | 30.04 | 35.44 | Positive |
| 11 | 15.2 | 35.33 | 37.47 | 26.37 | Positive | N/A | N/A | Negative |
| 12 | 9.0 | 36.01 | 40.09 | 24.87 | Positive | N/A | N/A | Negative |
| 13 | 2.8 | 37.53 | 39.62 | 25.27 | Positive | N/A | N/A | Negative |
| 14 | 1.7 | 38.2 | 40.12 | 26.97 | Positive | N/A | N/A | Negative |
| 15 | 1.2 | 38.58 | 39.99 | 27.74 | Positive | N/A | N/A | Negative |
| 16 | 0.2 | 41.15 | 39.51 | 27.98 | Positive | N/A | N/A | Negative |
| 17 | N/A | N/A | N/A | 23.11 | Negative | N/A | N/A | Negative |
| 18 | N/A | N/A | N/A | 26.6 | Negative | N/A | N/A | Negative |
| 19 | N/A | N/A | N/A | 25.56 | Negative | N/A | N/A | Negative |
| 20 | N/A | N/A | N/A | 25.12 | Negative | N/A | N/A | Negative |
| 21 | N/A | N/A | N/A | 25.26 | Negative | N/A | N/A | Negative |
| 22 | N/A | N/A | N/A | 27.63 | Negative | N/A | N/A | Negative |
| 23 | N/A | N/A | N/A | 26.35 | Negative | N/A | N/A | Negative |
| 24 | N/A | N/A | N/A | 25.6 | Negative | N/A | N/A | Negative |
| 25 | N/A | N/A | N/A | 27.77 | Negative | N/A | N/A | Negative |
| Sample | Conc. [copies/μL] | CT (2019-nCoV CDC EUA) |
Result (2019-nCoV CDC EUA) | CT (nCoV-QS) |
Result (nCoV-QS) | |||
|---|---|---|---|---|---|---|---|---|
| N1 | N2 | RP | ORF3a | N | ||||
| 1 | 883226.7 | 23.1 | 22.96 | 24.14 | Positive | 27.11 | 24.03 | Positive |
| 2 | 736560.0 | 23.31 | 23.39 | 27.04 | Positive | 27.51 | 24.39 | Positive |
| 3 | 153530.7 | 25.07 | 24.97 | 28.66 | Positive | 29.16 | 26.22 | Positive |
| 4 | 23205.6 | 27.2 | 27.48 | 27.63 | Positive | 31.22 | 28.19 | Positive |
| 5 | 4244.5 | 29.11 | 29.2 | 27.07 | Positive | 33.65 | 29.76 | Positive |
| 6 | 1886.7 | 30.02 | 30.88 | 29.81 | Positive | 34.23 | 30.84 | Positive |
| 7 | 1706.6 | 30.15 | 30.17 | 32.26 | Positive | 32.02 | 30.46 | Positive |
| 8 | 1573.9 | 30.13 | 30.12 | 31.17 | Positive | 32.26 | 30.53 | Positive |
| 9 | 719.5 | 31.1 | 31.2 | 26.25 | Positive | 35.4 | 31.03 | Positive |
| 10 | 176.0 | 32.69 | 32.77 | 24.04 | Positive | 26.45 | 31.87 | Positive |
| 11 | 143.5 | 32.8 | 32.36 | 31.46 | Positive | 35.09 | 32.81 | Positive |
| 12 | 51.6 | 34.07 | 34.51 | 26.56 | Positive | 39.43 | 39.89 | Positive |
| 13 | 40,3 | 34.35 | 35.39 | 24.38 | Positive | N/A | N/A | Negative |
| 14 | 3.3 | 37.17 | 37.43 | 26.51 | Positive | N/A | N/A | Negative |
| 15 | 1.8 | 37.87 | 38.27 | 30.89 | Positive | N/A | N/A | Negative |
| 16 | 1.6 | 37.63 | 37.62 | 29.84 | Positive | N/A | N/A | Negative |
| 17 | 0.6 | 39.05 | 38.8 | 27.27 | Positive | N/A | N/A | Negative |
| 18 | N/A | N/A | N/A | 28.25 | Negative | N/A | N/A | Negative |
| 19 | N/A | N/A | N/A | 27.07 | Negative | N/A | N/A | Negative |
| 20 | N/A | N/A | N/A | 30.45 | Negative | N/A | N/A | Negative |
| 21 | N/A | N/A | N/A | 30.65 | Negative | N/A | N/A | Negative |
| 22 | N/A | N/A | N/A | 27.92 | Negative | N/A | N/A | Negative |
| 23 | N/A | N/A | N/A | 29.59 | Negative | N/A | N/A | Negative |
| 24 | N/A | N/A | N/A | 29.09 | Negative | N/A | N/A | Negative |
| 25 | N/A | N/A | N/A | 30.16 | Negative | N/A | N/A | Negative |
| 26 | N/A | N/A | N/A | 30.19 | Negative | N/A | N/A | Negative |
| 27 | N/A | N/A | N/A | 30.76 | Negative | N/A | N/A | Negative |
| 28 | N/A | N/A | N/A | 31.06 | Negative | N/A | N/A | Negative |
| 29 | N/A | N/A | N/A | 33.76 | Negative | N/A | N/A | Negative |