Table 1.
Frequency of immune cells in tumor-bearing bladders.
| CD3+CD8+ | CD3+CD4+ | B cells | DCs | Macrophages | Neutrophils | NK | NKT | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |
| Untreated | 1.847 | 0.8701 | 4.493 | 0.8064 | 4.097 | 0.8173 | 0.5567 | 0.2173 | 50.6 | 7.374 | 5.103 | 0.7823 | 2.217 | 0.08 | 1.805 | 0.755 |
| f1994 | 4.737 | 2.665 | 5.69 | 1.114 | 5.35 | 1.074 | 0.5833 | 0.0702 | 44.57 | 5.404 | 8.6* | 0.7119 | 1.983 | 0.98 | 2.123 | 0.989 |
| aPD-1 | 6.09* | 1.472 | 8.757* | 1.862 | 7.15 | 2.405 | 0.78 | 0.0866 | 43.13 | 3.758 | 11.7* | 3.671 | 2.331 | 1.44 | 2.353 | 1.1124 |
| aPD-1+Cl994 | 5.287* | 1.04 | 6.437 | 1.752 | 6.697 | 3.116 | 0.96 | 0.3804 | 47.47 | 4.994 | 8.753 | 2.296 | 2.447 | 0.89 | 2.024 | 1.07 |
Bladders from treated mice were surgically resected, dissociated and prepared for flow cytometry analysis as shown in Figure 6. The frequency (the % positive cells of a specific phenotype among 10,000 events) of CD3, CD4, CD8, NK, NK T, B cells, DCs, macrophages, and neutrophils are shown. Statistical significance was calculated by student t-test,
*
P < 0.05. Bold indicates statistical significance.