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. 2020 May 15;11:974. doi: 10.3389/fimmu.2020.00974

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Copyright © 2020 Veerati, Troy, Reid, Li, Nichol, Kaur, Maltby, Wark, Knight, Bosco, Grainge and Bartlett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chemokine, cytokine and MUC5AC responses in differentiated BECs following RV-A1 infection. Quantification of chemokines (CXCL10, CCL5, IL-8) (A), cytokine (IL-6) and MUC5AC (B) protein levels in apical culture supernatants from BECs at 96 hpi. CXCL10, CCL5, and IL-6 were quantified by cytometric bead array and IL-8 and MUC5AC by ELISA. Cellular MUC5AC protein visualized by staining with anti-MUC5AC antibody (C), Scale bar: 25 μm. Biological replicates are represented as individual points (n = 5) and analyzed using Wilcoxon signed-rank test within the group (*p < 0.05) and between groups using Kruskal-Wallis test.