Figure 4.

Delayed induction of DEGs and molecular drivers. DEGs (FDR < 0.05; Fold change > 1.5) were identified at each timepoint following RV-A1 infection of healthy donor cultures and cultures from asthma or COPD donors (A). Data analysis was performed using EdgeR. Molecular drivers of gene expression changes were inferred using Upstream Regulator Analysis in healthy donor cultures (B). The top ten drivers presented are ranked by activation Z score. The dashed vertical line indicates FDR < 0.05. Red bars indicate pathway activation (activation Z-score > 2.0) and blue bars indicate pathway inhibition (activation Z-score < −2.0). (C) Heatmap (i) and PCA (ii) of DEGs across the time course of infection. Virus infected samples were normalized to their respective mock infected controls at each timepoint.