Fig. 4.
A: Analysis of the melting curves of the OGG1, NEIL1 and β-actin (housekeeping) genes: The coefficient of variations (% CV) for the expressions of the genes NEIL1, OGG1 and β-actin were 0.6, 1.2 and 1.5, respectively. B: Expression profiles of OGG1 and NEIL1: OGG1 (2-ΔΔCt) 0.43 (↓) (p = 0.007) and NEIL1(2-ΔΔCt) 0.01 (↑) (p = 0.489). The results are adjusted by age, gender, smoking status using lineer regression. 2-ΔΔCt (fold change) is computed using α = 0.016. Ct: cycle of threshold; ΔCt: the difference of Ct between target gene and β-actin (housekeeping) gene.