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. 2020 May 19;11(20):1799–1815. doi: 10.18632/oncotarget.27546

Figure 3. Entinostat augments cytotoxicity and cytokine production in an NK cell-intrinsic manner.

Figure 3

(A) Effect of entinostat pretreatment on NK degranulation as measured by surface CD107a after 6-hour coculture with an Ewing sarcoma tumor line (A-673). Each scatter plot represents a single tumor pretreatment condition, either DMSO or entinostat. (B) Effect of entinostat pretreatment on NK degranulation as measured by surface CD107a after 6-hour coculture with a rhabdomyosarcoma cell line (RD). Each scatter plot represents a single tumor pretreatment condition, either DMSO or entinostat. (C) Effect of entinostat pretreatment on intracellular IFN-γ production following 6-hour coculture with A-673. Each scatter plot represents a single tumor pretreatment condition, either DMSO or entinostat. (D) Effect of entinostat pretreatment on intracellular IFN-γ production following 6-hour coculture with RD. Each scatter plot represents a single tumor pretreatment condition, either DMSO or entinostat. Data shown in (A–D) are shown as percent positive cells obtained by treating purified NK cells from five to seven healthy donors per group. Blue and orange dots display NK pretreatment with DMSO and entinostat, respectively. Degranulation and IFN-γ production experiments were analyzed using one-way ANOVA with Geisser-Greenhouse correction of the four different experimental comparisons. Post hoc analysis of each pair of experimental comparison was completed using the paired t-test. * p < 005; ** p < 0.01.