Table 3. List of secondary antibodies and counterstaining of nuclei.

Secondary detection system used	Host	Method	Dilution	Supplier
Anti-Mouse-IgG (H+L)-HRP	Goat	WB	1:10,000	Sungene Biotech, China, Cat. #LK2003
Anti-Rabbit-IgG (H+L)-HRP	Goat	WB	1:10,000	Sungene Biotech, China, Cat. #LK2001
Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor® 488 Conjugate)	Goat	IF	1:50	Cell Signaling Technology, USA, cat. no. 4412
Hoechst 33342 (1 mg/ml) nucleic acid staining (DAPI)	-	IF	1:750	Molecular Probes/Invitrogen, Carlsbad, CA, USA, cat. no. A11007