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a Imidazolinone resistant mutants emerged with pooled T4 seeds were subject to MS medium with imazapic at 0.24 mg/l. b Genotypes of imidazolinone resistant mutants by Sanger sequencing of PCR amplicons. R in red stands for the heterozygosity of base A and G. A in red is homozygous edits. c Imidazolinone resistant alleles revealed by PCR amplicon cloning. Letters in green are modified nucleotides or amino acids.
