Table 1. Diverse base editing events generated at T2 generation using EC promoter.
T1 lines | T2 genotype | Editing ratio |
---|---|---|
1# | ATGATCCCGAGTGGTGGCAC | 2/6 |
ATGATCCCAGTGTGCAC | 1/6 | |
ATGATCCCGAGTGGTGCAC | 3/6 | |
2# | ATGATCCCGAGTGGTGGCAC | 5/12 |
ATGATCCCGAGTGGTGCAC | 3/12 | |
ATGATCCCGAGTGTGGCAC | 1/12 | |
ATGATCCCGAGTGTGCAC | 2/12 | |
ATGATCCCGAGTGTGCAC | 1/12 | |
3# | ATGATCCCGAGTGGTGGCAC | 15/18 |
ATGATCCCGAGTTGGCAC | 1/18 | |
ATGATCCCGAGTGTGCAC | 1/18 | |
ATGATCCCAGTGTGGCAC | 1/18 | |
4# | ATGATCCCGAGTGGTGGCAC | 12/14 |
ATGATCCCGAGTGGTGCAC | 1/14 | |
ATGATCCCGAGTGTGCAC | 1/14 |
R in red stands for the heterozygosity of base A and G. A in red is homozygous edits. 5’CCG3’ in blue is PAM.