Table 2. The genotype of T3 generation was influenced by re-editing of CBE and Mendel inheritance.
Lines | Genotype of | Genotype of | Genotype |
---|---|---|---|
T2 generation | T3 generation | frequency | |
901E-1-6 | CCAGTGGTGGC | CCAGTGGTGGC | 6/12 |
CCAGTGGTGGC | 1/12 | ||
CCGAGTGTGGC | 1/12 | ||
CCGAGTGGTGC | 1/12 | ||
CCAGTGGTGC | 2/12 | ||
901E-1-7 | CCGAGTGGTGC | CCGAGTGGTGC | 2/9 |
CCGAGTGGTGC | 7/9 | ||
901E-1-8 | CCGAGTGGTGC | CCGAGTGGTGC | 8/10 |
CCGAGTGGTC | 1/10 | ||
CCGAGTTGC | 1/10 | ||
901E-13-22 | CCGAGTGGTGC | CCGAGTGGTGC | 5/8 |
CCGAGTGGTGC | 3/8 | ||
901E-13-16 | CCGAGTGGTGC | CCGAGTGGTGC | 3/8 |
CCGAGTGGTGC | 3/8 | ||
CCGAGTGTGGC | 2/8 | ||
901E-1-3 | CCGAGTGTGC | CCGAGTGTGGC | 1/7 |
CCGAGTGGTGC | 1/7 | ||
CCGAGTGGTGC | 1/7 | ||
CCGAGTGATGC | 1/7 | ||
CCGAGTGTGC | 3/7 | ||
901E-1-4 | CCGAGTGTGC | CCGAGTGTGC | 8/9 |
CCGAGTGTGC | 1/9 | ||
901E-1-16 | CCAGTGTGC | CCAGTGTGC | 6/9 |
CCAGTGTGC | 1/9 | ||
CCAGTGTGC | 1/9 | ||
CCAGTGTGC | 1/9 |
R in red stands for the heterozygosity of base A and G. A in red is homozygous edits.