Figure 5.
Intrinsic plasticity, but not synaptic plasticity, was impaired in STIM1PKO. A, Plots of the normalized EPSC change after application of LTD induction protocol in WT littermates (n = 8, n = 4 after 40 min, blue) and STIM1PKO mice (n = 7, n = 6 after 40 min, red). Synaptic plasticity was normally induced in both genotype groups. Calibration: 200 pA, 50 ms. B, Bar graphs represent the comparison of excitability changes after LTD induction. In STIM1PKO mice, downregulation of excitability in PCs was shown 20 min after induction (n = 7, p = 0.009); however, it had fully recovered 40 and 50 min after induction (40 min, n = 7, p = 0.286; 50 min, n = 6, p = 0.690). On the contrary, the intrinsic plasticity was induced and slightly further developed in time from WT littermates (20 min, n = 8, p < 0.001; 40 min, n = 8, p < 0.001; 50 min, n = 4, p < 0.001). Calibration: 20 mV, 200 ms. B, One-way repeated-measures ANOVA with post hoc Dunnett correction. *p < 0.05; ***p < 0.001. n.s., not significant.