Figure 7.

After rank-ordering the control and TTX data, a plot of TTX mEPSC/control mEPSC as a function of control mEPSC shows a roughly constant ratio for simulated uniform multiplicative scaling, a predictable deviation over the initial portion of the data for simulation of a detection threshold, but a non-constant ratio over a large portion of the data range for the experimental data. A, For simTTX/ranCON1, the ratio is close to the expected value of 1.31 (dashed vermilion line). B, For data truncated by a 5-pA detection threshold, the ratio simTTX/ranCON1 is underestimated for a small range of data in the range of the 5-pA threshold, but is otherwise close to the expected value (dashed vermilion line). C, For data truncated by a 7-pA detection threshold, the ratio is underestimated for the bottom 25% of the data, then reaches a plateau; the plateau systematically underestimates the expected value thereafter (dashed vermilion line). D, For experimental data the ratio increases smoothly until reaching a plateau halfway between the 50th and 75th percentiles; the ratio is first under and then surpasses the slope factor estimated from linear regression of the rank-ordered data (dashed vermilion line). Solid blue lines indicate 25th, 50th, and 75th percentiles. Dashed blue lines indicate the simulated 5-pA (B) and 7-pA (C) thresholds, and the 3-pA detection threshold used for experimental data (D).