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. 2020 May 22;6(21):eaay9320. doi: 10.1126/sciadv.aay9320

Fig. 5. Rational mutagenesis for reductive amination with aniline.

Fig. 5

(A) Comparisons of theoretical extended active site residues for the reductive amination of cyclohexanone with aniline in AspRedAm to the analogous residues in the novel IREDs p-IR23 and p-IR48. (B) Conversions in the reductive amination of cyclohexanone with aniline with purified wild-type p-IR23, wild-type p-IR48, p-IR48 F246L, and p-IR48 T172S/M178L/L182M/F246L at different time points. Conversions were determined by GC-FID with comparison to a chemically synthesized standard. The adjacent image shows the positions of nonconserved residues with 6 docked in the active site, based on homology modeling. Residue 244* is attached to the second protein chain in the dimer.