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. 2020 May 22;6(21):eaaz1261. doi: 10.1126/sciadv.aaz1261

Fig. 2. Most of the germ cells do not respond to ATRA before 13.5 dpc.

Fig. 2

(A) Immunodetection of ATRA-responsive cells (GFP positive, green) and germ cells (POU5F1- or TRA98-positive cells, red) in 10.5, 11.5, and 13.5 dpc Tg(RARE-Hspa1b-cre/ERT2) ovarian sections after TAM induction at 9.5, 10.5, and 12.5 dpc, respectively. DAPI (blue), nuclei. Scale bars (white), 50 μm. (B) Immunodetection of ATRA-responsive cells (GFP, green) and TRA98 (germ cells, red) in 14.5 dpc Tg(RARE-Hspa1b-cre/ERT2) whole ovaries after TAM induction at 13.5 dpc. Scale bars (white), 50 μm. White arrowheads, GFP-positive germ cells. (C) Immunodetection of TRA98 (germ cells) (red) and GFP (ATRA-responsive cells) (green) in the presence of either DMSO (control) or 1 or 100 nM ATRA in cultured Tg(RARE-Hspa1b-cre/ERT2) ovaries from 13.5 to 14.5 dpc. Asterisk, GFP-positive cells within the mesonephros.