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. 2020 May 22;6(21):eaax3333. doi: 10.1126/sciadv.aax3333

Fig. 6. LysRS-mediated negative regulation of Ifnβ expression is conserved in zebrafish.

Fig. 6

(A) WB analysis of pull-down experiments performed as in Fig. 2D, except that 1, 3, and 10 pmol of recombinant zebrafish LysRS (zlysrs) protein were used; (n = 3). (B) Schematic representation of the zebrafish lysrs locus. The region targeted by lysrs-targeting morpholino oligo (MO-lysrs) and primers used for amplification of lysrs are indicated by a red line or black arrows, respectively. Boxes, exons; straight lines, introns. (C) Larvae injected with MO-lysrs or with control MO (MO-Ctl). (D) lysrs, ifnφ1, and isg15 mRNA levels were quantified by RT-qPCR in larvae injected with MO-lysrs or with MO-Ctl. Median (±SEM) of 12 pools of three independent larvae per condition. Unpaired t test. ***P < 0.001, ****P < 0.01. All blots show representative experiments.