Table 1.

Sequences of the Primers (Carbapenemase Genes and Other β-Lactamase Genes)

Target Gene	Primer	Primer Sequence (5′–3′)	Product Size (bp)	Annealing Temperature (°C)
KPC	KPC-F	TGTCACTGTATCGCCGTC	1010	58
	KPC-R	CTCAGTGCTCTACAGAAAACC
IMP1	IMP1-full-F	TGAGCAAGTTATCTGTATTC	740	55
	IMP1-full-R	TTAGTTGCTTGGTTTTGATG
IMP2	IMP2-full-F	GGCAGTCGCCCTAAAACAAA	737	55
	IMP2-full-R	TAGTTACTTGGCTGTGATGG
NDM	NDM-full F	ATGGAATTGCCCAATATTATGCAC	816	61
	NDM-full R	TCAGCGCAGCTTGTCGGC
VIM1	VIM1-full-F	TTATGGAGCAGCAACCGATGT	920	55
	VIM1-full-R	CAAAAGTCCCGCTCCAACGA
VIM2	VIM2-full-F	AAAGTTATGCCGCACTCACC	865	55
	VIM2-full-R	TGCAACTTCATGTTATGCCG
OXA-48	OXA-48-F	GCGTGGTTAAGGATGAACAC	438	52
	OXA-48-R	CATCAAGTTCAACCCAACCG
CTX-M1 group	CM3-full-F	TTTCGGAAGCATAAAATCGG	1021	56
	CM3-full-R	GGCGATAAACAAAAACGGAA
CTX-M2 group	P3-F	ATGATGACTCAGAGCATTCG	832	65
	P2b-R	TCCCGACGGCTTTCCGCCTT
CTX-M3 group	YW3459-F	AAAAATGATTGAAAGGTGGT	1242	56
	YW3460-R	GTGAAGAAGGTGTTGCTGAC
DHA	DHA1-F	CTGATGAAAAAATCGTTATC	1141	56
	DHA1-R	ATTCCAGTGCACTCAAAATA
CMY	CMY-F	TGTCAACACGGTGCAAATCA	1346	56
	CMY-R	AGCAACGACGGGCAAAATG
ACT	ACT-F	CGAACGAATCATTATTCAGCACCG	1518	56
	ACT-R	CGGCAATGTTTACTACACAGCG