KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Pard6a (clone C-3) (1:100) | Santa Cruz Biotechnology | Cat# sc-365323, RRID:AB_10846183 |
| Rabbit monoclonal anti-Integrin alpha 6 (clone EPR18124) (1:200) | Abcam | Cat# ab181551 |
| Rabbit polyclonal anti-Fak (1:200) | Abcam | Cat# ab131435, RRID:AB_11154758 |
| Rabbit monoclonal anti-Phospho-FAK (Tyr397) (clone 31H5L17) (1:200) | Thermo Fisher Scientific | Cat# 700255, RRID:AB_2532307 |
| Rabbit polyclonal anti-Zeb1 (1:1000) | Sigma-Aldrich | Cat# HPA027524, RRID:AB_1844977 |
| Rabbit monoclonal anti-Bnip3/Nix (clone D4R4B) (1:500) | Cell Signaling Technology | Cat# 12396, RRID:AB_2688036 |
| Rabbit polyclonal anti-alpha internexin (1:1000) | Abcam | Cat# ab7259, RRID:AB_305807 |
| Goat polyclonal anti-GFP (1:500) | Abcam | Cat# ab6673, RRID:AB_305643 |
| Mouse monoclonal anti-activated beta1 integrin HUTS4 | Millipore | Catalog #: MAB2079Z |
| Rabbit polyclonal anti-Hif1 alpha (1:100) | ThermoFisher | Cat# PA1–16601 RRID:AB 2117128 |
| Mouse monoclonal anti-Human Ki-67 (1:50) | BD Pharmingen | Cat# 556003 RRID:AB_396287 |
| Rabbit polyclonal anti-Glut1 (1:500) | Millipore | Cat# 07–1401 RRID:AB_1587074 |
| Mouse monoclonal anti-p27 Kip1 (1:100) | Cell Signaling | Cat# 3698 RRID:AB_2077832 |
| Rabbit polyclonal anti-phospho Histone H3 (1:500) | Millipore | Cat# 06–570 RRID:AB_310177 |
| Rabbit polyclonal anti-Caspase 3 (1:100) | Cell Signaling | Cat# 9664 RRID:AB_2070042 |
| Mouse polyclonal anti-CHL-1 (1:100) | R&D Systems | Cat# AF2147 RRID:AB_2079332 |
| Mouse monoclonal anti-NeuN (1:100) | Millipore | Cat# MAB377 RRID:AB_177621 |
| Rabbit polyclonal anti-Partitioning-defective 3 (1:100) | Millipore | Cat# 07–330 RRID:AB_11213581 |
| Chicken polyclonal anti-GFP (1:100) | Aves Labs, Inc | Cat# GFP-1020 RRID:AB_10000240 |
| Hoeschst 33342 Staining Dye/Dapi (1:500) | Abcam | Cat# ab228551 |
| Donkey anti-chicken 649 (1:500) | Jackson Immuno Labs | Cat# 703-606-155 RRID:AB 2340380 |
| Donkey anti-rabbit 555 (1:500) | Invitrogen | Cat# A31572 RRID:AB_162543 |
| Donkey anti-mouse 488 (1:750) | Invitrogen | Cat# A32766 RRID:AB_2762823 |
| Donkey anti-rabbit 488 (1:750) | Invitrogen | Cat# A32790 RRID:AB_2762833 |
| Donkey anti rabbit 649 (1:750) | Invitrogen | Cat# A32795 RRID:AB_2762835 |
| Donkey anti-mouse 555 (1:750) | Invitrogen | Cat# A32773 RRID:AB_2762848 |
| Click-IT Edu AlexaFluor 488 Imaging Kit | Invitrogen | Cat# C10337 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Echinomycin | Tocris | Cat# 5520 |
| Mouse Laminin | EMD Millipore, Chemicon | Cat# CC095 |
| Gant 61 | Tocris | Cat# 3191 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Microarray Data: ArrayExpress E-MTAB-8761 | E-MTAB-8761 | |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Mouse: Atoh1-eGFP: B6.129S-Atoh1tm4.1Hzo/J | The Jackson Laboratory | JAX: 013593 |
| Mouse: Ptch1: B6N.129-Ptch1tm1Hahn/J | The Jackson Laboratory | JAX: 012457 |
| Mouse: Hif1a: B6.129-Hif1atm3Rsjo/J | The Jackson Laboratory | JAX: 007561 |
| Mouse: Vhl: B6.129S4(C)-Vhltm1Jae/J | The Jackson Laboratory | JAX: 012933 |
| Mouse: Egln1: Egln1tm1Kael/J | The Jackson Laboratory | JAX: 009672 |
| Mouse: Itgb1: B6;129-Itgb1tm1Efu/J | The Jackson Laboratory | JAX: 004605 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| pCIG2 Histone2B-mCherry | Laboratory Stocks | |
| pCIG2 Inactive Cre T2A Histone 2B mCherry | “ | |
| pCIG2 Active Cre T2A Histone 2B mCherry | “ | |
| pCIG2 HifaHD cDNA | “ | |
| pCIG2 Zeb1 cDNA | “ | |
| pCIG2 Pard6α cDNA and point mutants | ||
| pCIG2 Pard3A cDNA | “ | |
| pCIG2 Chl1 cDNA | “ | |
| pCIG2 mir30 Zeb1 shRNA | “ | |
| pCIG2 mir30 Pard3A shRNA | “ | |
| pCIG2 mir30 Pard6 α shRNA | “ | |
| Software and Algorithm Section | ||
| Image Studio 5.2 | Biotechnology |
https://www.licor.com/bio/image-studio/, RRID:SCR_015795 |
| Amira | ThermoFisher | https://www.thermofisher.com/us/en/home/industrial/electron-microscopy/electron-microscopy-instruments-workflow-solutions/3d-visualization-analysis-software/amira-life-sciences-biomedical.html |
| Slidebook | Intelligent Imaging Innovations | https://www.intelligent-imaging.com/slidebook |