Fig. 3.

Role of fluoxetine-induced D1 receptors in adult neurogenesis in the dentate gyrus. a Experimental design for treatment of mice with placebo (PL) or fluoxetine (FLX; 15 mg/kg/day for 14 days) pellet and injection of R(+)-SKF81297 (SKF; 1.5 mg/kg/day i.p. for 5 days), SCH23390 (SCH; 0.1 mg/kg/day i.p. for 14 days) or saline (SAL) and for neurogenesis analysis with BrdU injection and perfusion (P). On day 15, mice were perfused 2 h after BrdU injection to evaluate cell proliferation. b, c Immunostaining with BrdU and doublecortin antibodies in the dentate gyrus (b) and quantitation of BrdU-positive cells in the subgranular zone of the dentate gyrus on both sides in placebo or fluoxetine pellet-treated mice (n = 7–8) (c). Scale bars, 100 µm. **p < 0.01 vs. the placebo/saline group; ***p < 0.001 vs. the placebo/SKF81297 group; two-way ANOVA and Bonferroni post hoc test. ^#p < 0.05 vs. the fluoxetine/saline group; ^##p < 0.01 vs. the fluoxetine/SKF81297 group; one-way ANOVA and Newman−Keuls post hoc test