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. 2018 Dec 10;25(6):1229–1244. doi: 10.1038/s41380-018-0316-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Role of D1 receptors on behavioral effects of chronic fluoxetine treatment. a−e Effects of treatment with the placebo (PL) or fluoxetine (FLX; 15 mg/kg/day for 14 days) pellet on the latency to feed in the novelty-suppressed feeding test (NSFT) (b) and the immobility time in the tail suspension test (TST) (c). After the behavioral tests, the expression levels of Drd1 mRNA (d) and protein (e) in the dentate gyrus were evaluated. *p < 0.05 vs. the placebo group; Student’s t test. f−j AAV vectors (AAV-DIO-Drd1-P2A-mCherry or AAV-DIO-YFP) were injected into both sides of the dentate gyrus of [Drd1]-Cre mice to overexpress D1 receptors. An image of the dentate gyrus obtained on the same day of needle insertion showed that the edge of injection needle (arrow head) was in the right place. mCherry was mainly expressed in NeuN-positive granule cells of the dentate gyrus after injection of AAV-DIO-Drd1-P2A-mCherry (f). ML molecular layer, GC granule cell layer, H hilus. Scale bars, 100 µm. Overexpression of D1 receptors in the dentate gyrus was confirmed by measurements of mRNA (g) and protein (h) levels. D1 receptor overexpression in the dentate gyrus decreased the latency to feed in the NSFT (i) and the immobility time in the TST (j). Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 vs. the control (C) group; Student’s t test. The number of mice is indicated in parentheses under each experimental condition