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. 2020 May 18;15:3523–3537. doi: 10.2147/IJN.S237008

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© 2020 Yang et al.

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High-glucose conditions inhibited osteogenesis on different modified Ti surfaces. (A) The Alizarin Red staining on different modified Ti surfaces after osteogenic induction for 21 days under high-glucose conditions. (B) Semi-quantitative analysis of the Alizarin Red staining for 21 days. High-glucose conditions inhibited the mineralization level on different modified Ti surfaces. The TNT surface could alleviate the inhibition of mineralization when compared with the SLA surface under high-glucose conditions. The Alizarin Red staining for 14 days on different modified Ti surfaces is shown in Figure S1. **p < 0.01, ***p < 0.001.