Figure 2.

Inhibition of necroptosis alleviates CSE-induced mRNA transcripts of inflammatory cytokine in BMDMs. BMDMs were incubated with 1%CSE for 24h in the absence or presence of the indicated necroptosis inhibitor. Relative mRNA levels of CXCL1, CXCL2 and IL6 were detected by qRT-PCR (A–F). Data are mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 (Student’s t-test).

Abbreviations: CSE, cigarette smoke extract; BMDMs, bone marrow–derived macrophages; NEC-1, necrostatin-1; qRT-PCR, quantitative real-time polymerase chain reaction; SEM, standard error of the mean.