Fig. 3.

Phenotypic and genotypic screening of S. lividans colonies after conjugation with pCMU-4dGly and induction with a 4 mM, c 8 mM and e 12 mM of theophylline. PCR verification with set of primers ‘dGly’ and ‘SCO1660 check’ of colonies after induction with b 4 mM, d 8 mM and f 12 mM of theophylline. Positive recombinants are shown in red