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. 2020 May 4;47(4):413–423. doi: 10.1007/s10295-020-02277-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Phenotypic and genotypic screening of S. coelicolor exconjugants after conjugation with pCM4.4-Act-KO, pCM4.4. a Image of conjugation plates after 5 days of incubation at 30 °C. pCM4.4-Act-KO exconjugants do not show actinorhodin (blue pigment) production. b Re-streaked exconjugants for both pCM4.4 and pCM4.4-Act-KO confirm lack of actinorhodin production for edited exconjugants. c Genotypic screening of 8 exconjugants confirm deletion of 852 bp target for 7 colonies