Table 2.
Number of exconjugantsa and percentage of survivalb from Cas9 tolerance experiments
| Plasmid | Theophylline concentration (mM) | |||
|---|---|---|---|---|
| 0 | 0.5 | 1 | 2 | |
| Streptomyces coelicolor M145 | ||||
| pCM2.1 | 0 | N/A | N/A | N/A |
| pCM(-cas9) | 10,140a/100b | 5,640/55 | 6,000/59 | 1,800/17 |
| pCMU | 13,200/100 | 8,208/62 | 7,044/53 | 3,780/28 |
| pCMUtuf | 0 | 0 | 0 | 0 |
| pCMU-4 | 11,760/100 | 8,124/69 | 5,712/49 | 1,440/12.2 |
| pCMU-4tuf | 10,560/100 | 36/0.003 | 0 | 0 |
| Streptomyces lividans TK24 | ||||
| pCM2.1 | 0 | N/A | N/A | N/A |
| pCM(-cas9) | 19,320/100 | 16,920/88 | 12,960/67 | 3,276/17 |
| pCMU | 0 | 0 | 0 | 0 |
| pCMUtuf | 0 | 0 | 0 | 0 |
| pCMU-4 | 18,000/100 | 12,516/70 | 9,516/53 | 1,560/9 |
| pCMU-4tuf | 20,304/100 | 5,760/28 | 3,480/17 | 240/0.01 |
aNumber of exconjugants expressed as number of colony-forming units (CFUs) per conjugation
bPercentage of survival considering number of CFUs in the uninduced condition (0 mM theophylline) as 100%