Fig. 6. Strand invasion, meiotic crossover, and recombination are impaired in Ppp6c^cKO spermatocytes.

a Spermatocytes stained for SYCP3 (red) and DMC1 (green) in Ppp6c^WT and Ppp6c^cKO 8-week-old mice. Scale bar: 10 μm. b Quantification of DMC1 foci in leptotene stage and zygotene stage (n = 3; 100 cells). c Spermatocytes stained for SYCP3 (red) and MLH1 (green) in Ppp6c^WT and Ppp6c^cKO 8-week-old mice. MLH1 is a recombination marker in Ppp6c^WT spermatocytes and decreased MLH1 staining was observed in Ppp6c^cKO mice spermatocytes Scale bar: 10 μm. d MLH1 signals were counted in spreads. Quantification of the MLH1 foci numbers per cell in the Ppp6c^WT and Ppp6c^cKO spermatocytes (n = 3; 100 cells). e Immunoblotting analysis of MRE11, DMC1 in Ppp6c^WT, and Ppp6c^cKO germ cells (12 dpp). Level of β-actin was detected as internal control. Each experiment was repeated at least three times.