Figure 5.
G protein signaling of diverse DOP receptor agonists. AtT-20 cells stably expressing HA-hDOP receptors were stimulated with vehicle, or DPDPE, deltorphin I (A), SNC80 (B), ADL5859 (C), AR-M1000390 (D), fentanyl (E) or morphine (F) at a concentration range of 10−6 to 10−13 M and GIRK channel activation was measured in a membrane potential assay. Dose response curves were calculated with OriginPro using sigmoidal non-linear fitting for data from three independent experiments performed in duplicate (mean ± SEM). Vehicle-induced changes in fluorescence signal (background) were subtracted from signals obtained using agonist-containing solutions. The DPDPE graph is the same in every panel and serve as a comparator. RFU, relative fluorescence units.