Fig. 5. SetCys redox switch enables the straightforward synthesis of linear and cyclic proteins in one-pot.

a Schematic representations of peptidic N- and C-ter extremities. b One-pot grafting of a peptide linker to a unique linear precursor yields cyclic polypeptides. Application to the total synthesis of cyclic and biotinylated analogs of HGF/SF K1 domain (cK1-1, cK1-2). c One-pot synthesis of linear K1 domain.