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. 2020 May 22;10:8548. doi: 10.1038/s41598-020-65464-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overall setup for optical mapping of the whole pig heart in situ. (a) Whole optical mapping setup adapted for registration of the cardiac electrical activity of the pig heart in situ during open-heart surgery. (b) Heart with inserted cannulas. (c) Hand-made frame for heart immobilization. Arrow indicates the bar. (d) Octopus tissue stabilizer used for heart immobilization. (e) Wheel with 8 positions for excitation LEDs (four 660 nm and four 780 nm).