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. 2020 May 22;10:8548. doi: 10.1038/s41598-020-65464-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrical activity in the pig heart recorded by optical mapping in situ using the fluorescent dye di-4-ANBDQBS under artificial blood circulation. Activation time, conduction velocity and vector maps were obtained during pacing at four different locations, i.e., the septum (a), left ventricular endocardium (LV endo) (b), left ventricular epicardium (LV epi) (c), and right ventricular epicardium (RV epi) (d), with a 500-ms period. The numbers near the isochrones show the activation time in ms in the activation maps and the conduction velocity in m/s in the conduction velocity maps. The interval between isochrones is 10 ms for the activation time and 0.2 m/s for the conduction velocity.