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. 2020 May 22;10:8548. doi: 10.1038/s41598-020-65464-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrical activity in the pig heart in situ recorded by optical mapping using the fluorescent dye Cardiogreen at various excitation and emission wavelengths during apex pacing with a 500-ms period under artificial blood circulation. The maps of the activation time and OAPs from the sites indicated by asterisks in the activation map in the regions of the right ventricle (RV), artery (Ar), and left ventricle (LV), obtained at λ_ex = 660 nm and λ_em = 775 nm, are presented in the top row. The maps of the activation time, OAPD50 and repolarization time and the OAPs from the sites indicated by asterisks in the activation maps obtained at λ_ex = 780 nm and λ_em = 808 nm are presented in the middle row. The activation time, OAPD50 and repolarization time maps and the OAPs constructed from the OAPs calculated as the ratio of the two OSs obtained at λ_ex = 660 nm/λ_em = 720 nm and λ_ex = 780 nm/λ_em = 808 nm are presented in the bottom row. The numbers near the isochrones show the activation time, OAPD50 and repolarization time in ms. The interval between isochrones is 5 ms for the activation time and 10 ms for the OAPD50 and repolarization time. The amplitude of the OAPs is given as a percentage with respect to the background (ΔF/F).