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. 2019 Dec 9;27(6):1844–1861. doi: 10.1038/s41418-019-0467-6

Fig. 3. UPF1 is required for the association between Linc-ASEN and PRCs, and is involved in histone modification of the p21 gene locus.

Fig. 3

a ChIRP was performed using Linc-ASEN specific biotin-probes. Enrichment of DNA was quantified relative to the amount of input. b Relative Linc-ASEN levels in the nucleus and cytoplasm were assessed using nucleus and cytoplasm extraction assay after transfection of UPF1 Si. c, d H3K27me3 ChIP was performed in MCF7 cells transfected with Linc-ASEN Si (c) or UPF1 Si (d). H3K27me3 enrichment at the p21, NOXA and GADD45A promoters were determined by ChIP-qPCR analysis. Primer pairs were selected in the p53 responsive region. A rabbit IgG (rIgG) was used as a negative control. e, f ChIP was performed by H3K4me3 (e) and H3ac (f) antibodies in Linc-ASEN silenced MCF7 cells. DNA in the precipitates was measured using each specific primer and quantified relative to the amount of input. rIgG antibody was used as a negative control. In a, c–f, data are represented as means ± SD. *P < 0.05, **P < 0.01, #P > 0.05 (Student’s t-test).