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. 2019 Dec 9;27(6):1844–1861. doi: 10.1038/s41418-019-0467-6

Fig. 4. Linc-ASEN physically binds to p21 gene locus with complementary base pairing.

Fig. 4

a Integrated Genome Viewer (IGV 2.3) showing the expression pattern of p21 in Linc-ASEN-depleted MCF7 cells, and peaks of Linc-ASEN ChIRP-Seq and UPF1 ChIP-Seq on the p21 gene locus. b Predicted motifs enriched in Linc-ASEN peaks using MEME (http://meme-suite.org). c Schematic Linc-ASEN-binding site on the p21 gene. The Linc-ASEN and UPF1 binding sites were analyzed by ChIRP-qPCR and ChIP-qPCR, respectively. Locations of each primer (primer 1~4) are shown in the schematic. d The rate of p21 transcription in MCF7 cells transfected with pCMV-Linc-ASEN or pCMV-Linc-ASEN Δp21 DBS construct was analyzed by RT-qPCR analysis. e MCF7 cells were transfected with pCMV-Linc-ASEN-MS2BS (Wt) or pCMV-Linc-ASEN Δp21 DBS-MS2BS reporter (Mut) plasmids in combination with pMS2-HA. IP was performed using HA antibody, and then precipitated DNA was quantified using specific primers. NC, negative control. In c–e, data are represented as means ± SD. *P < 0.05, **P < 0.01, #P > 0.05 (Student’s t-test).