Table 1.
Growth and inhibitory properties of the preliminary hit molecules.
| Preliminary Hits | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| Differential Growtha | 65.7 ± 0.5% | 96.2 ± 0.3% | 17.5 ± 3.5% | 38.5 ± 0.5% | 96.2 ± 0.3% | 71.8 ± 16.5% |
| MIC (MG-1)b | 2.0 | 0.5 | 8.2 | 16.4 | 1.0 | 2.1 |
| MIC (ΔgspA/ΔsrtA)b | 4.0 | 2.1 | >130 | >130 | 2.0 | 8.4 |
| IC50 (A. oris)c | >190 | >200 | 30 ± 20 | 70 ± 50 | >190 | 60 ± 40 |
| IC50 (S. aureus)c | 80 ± 20 | >200 | 90 ± 20 | 200 ± 100 | 110 ± 30 | 54 ± 8 |
aDifferential effects of small molecule on wild-type MG-1 and ΔgspAΔsrtA A. oris strains measured in the high-throughput screen. See methods section complete definition. bMinimum inhibitor concentration (MIC) expressed in μg ml−1 for MG-1 and ΔgspAΔsrtA A. oris strains. cHalf maximal inhibitory concentration for the in vitro enzyme activity of SaSrtA (S. aureus) or AoSrtA (A. oris) in μg ml−1.