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. 2020 May 22;11:2565. doi: 10.1038/s41467-020-16328-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representation of a suspended, rhodamine-labeled, template microtubule immobilized on optically transparent polymer ridges by anti-rhodamine antibodies. Atto647n-labeled transport microtubules are capable of freely accessing the 3D lattice of the template microtubule between the ridges (in the region referred to as valley), as they slide along them driven by GFP-labeled Ncd motors. b The perpendicular distance of the center point of the transport microtubule from the tracked center line of the template microtubule provides the sideways distance. Positive values are arbitrarily assigned to positions on the left. c Sideways distance for an example antiparallel transport microtubule as it slides along a template microtubule over two valley regions (see also Supplementary Movie 1). In both valley regions, the 1.8 μm long transport microtubule exhibited a helical motion around the suspended template microtubule, with a helical pitch of 1.3 ± 0.1 μm (N = 7 rotations, mean ± SD). At the ridge, where the template microtubule is surface-immobilized, the transport microtubule remained on the right-hand side of the template microtubule, indicating that the direction of helical motion is right-handed.