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. 2020 May 22;10:8509. doi: 10.1038/s41598-020-65397-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rapamycin inhibits decease in insulin-stimulated Akt pathway activation in exercised leg. (a) Representative Western blot images. The grouping of blots cropped from different parts of the same gel, or from different gels, fields, or exposures were divided by black lines. (b) Akt Th308, (c) Akt Ser473, (d) TBC1D1 Ser231, (e) TBC1D4 Set597 and (f) TBC1D4 Thr651 phosphorylation in response to insulin in CON or RE leg under treatment of either placebo or rapamycin. n = 6–8 in each group. Values are means ± standard error. *P < 0.05 versus placebo injection within CON or RE legs, ^♯P < 0.05 versus CON leg for each group, ^§P < 0.05 versus response to RE (interaction of RE × rapamycin). RE, resistance exercise; CON, unstimulated control.