Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 22;10:8542. doi: 10.1038/s41598-020-65381-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HN expression in human and murine breast tumor cells and tissues. HN expression was evaluated in human breast tumor cells MCF7, T47D and MDA-MB-231 (MDA) and in murine NMuMG, LM3 and 4T1 cells. (A) Representative histograms show the mean fluorescence intensity (MFI) of tumor cells, as assessed by flow cytometry. (B) Representative gel shows the expression of HN mRNA and internal control β-glucuronidase (GUS) or cyclophilin (CYP), as assessed by RT-PCR. (C) Images show the expression of HN in human (MDA-MB-231) and murine (4T1) TNBC cells, as evaluated by immunofluorescence. Images show HN (green), nuclei stained with DAPI (blue) and their overlay. (D) Representative microphotographs show HN expression as assessed by immunohistochemistry in paraffin sections of primary 4T1 TNBC tumors. Insets show cells expressing HN (left, arrows) and a control without primary antibody (right). N: necrotic area. (E) Low (left) and high (right) magnification images showing HN expression in metastatic nodules (M) in lungs from 4T1 TNBC-bearing mice. Insets show cells expressing HN (left, arrows) and a control without primary antibody (right). (F) Images show the expression of HN in murine (4T1) TNBC cells transfected with p.shHN, as evaluated by immunofluorescence. Images show HN-positive cells (red), citrine-positive (transfected cells, green), nuclei stained with DAPI (blue) and their overlay. Arrows indicate transfected cells that do not express HN. (G) Representative microphotographs show HN expression (green) as assessed by immunofluorescence in paraffin sections from mammary gland samples from healthy donors that underwent cosmetic breast surgery (left panel) and tumor biopsies from TNBC patients (right panel). Nuclei stained with DAPI (blue). The graph depicts the median fluorescence intensity (MFI) of HN immunofluorescence in samples of normal mammary gland (n = 5) or biopsies from breast tumor patients (n = 5), as analyzed with ImageJ software. To obtain the fluorescence intensity/field for each sample a mean value was calculated by assessing 20 fields.