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. 2020 May 22;11:2578. doi: 10.1038/s41467-020-16306-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–c The protein (a), mature mRNA (b), or precursor mRNA (c) of Mettl3 in ECT1/E6E7, HeLa, and SiHa cells were checked. d The promoter activities of Mettl3 in ECT1/E6E7, HeLa, and SiHa cells were checked by dual-luciferase assay. e Venn diagram shows the overlap of transcription factors of Mettl3 predicted by PROMO and ChIPBase, respectively. f The mRNA expression of potential transcription factors of Mettl3 in ECT1/E6E7, HeLa, and SiHa cells were checked by qRT-PCR analysis. g The protein expression of NRF1 and TBP was checked by western blot analysis (left) and quantitatively analyzed (right). h, i Cells were transfected by siRNA negative control (si-NC) or siRNAs of TBP for 24 h, the mRNA (h) and protein (i) levels of Mettl3 were checked. j The binding between TBP and promoter of Mettl3 was checked by ChIP-PCR using IgG or TBP antibody. k Binding between TBP transcriptional factor and the promoter of Mettl3 at the potential binding site 1 and 2 or negative site BLK was checked by ChIP-PCR. l Schematic representation of the mutated promoter in pGL3-Basic-Mettl3-luc reporter to investigate the role of TBP in Mettl3 expression. m HeLa cells were co-transfected with pGL3-Mettl3-WT-Luc, pGL3-Mettl3-Mut1-Luc, pGL3-Mettl3-Mut2-Luc, pRL-TK plasmid and si-NC or si-TBP-1 for 24 h. Results were presented as the ratio between the activity of the reporter plasmid and pRL-TK. n Correlation between Mettl3 and TBP in cervical cancer patients (n = 309) from ChIPBase database. Data are presented as the mean ± SD from three independent experiments. **p < 0.01. NS, no significant, by one-way ANOVA for (b) (p < 0.001 for all), (c) (p < 0.001 for all), (d) (p < 0.001 for all), (f, g) (p < 0.001 for all), and (h) (p < 0.001 for all), two-tailed unpaired Student’s t test for (j) (p < 0.001 for all), (k) (p < 0.001 for all), and (n) (p = 0.0005), and by two-way ANOVA for (m) (p < 0.001 for all).