Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 22;3:252. doi: 10.1038/s42003-020-0989-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a IHC staining for IDO1 (upper panel) and double ISH for Ido1 and Lgr5 mRNA (lower panel) in Stat1^flox/flox Apc^Min and Stat1^∆IEC Apc^Min colon tumors. IDO1⁺ cells are indicated by arrowheads. Scale bars indicate 20 µm. b Quantification of IDO1⁺ tumor cells in IHC-stained tumors of Stat1^flox/flox Apc^Min (si and colon: 13 tumors, 4 mice) and Stat1^∆IEC Apc^Min (si: 12 tumors, 5 mice; colon: 13 tumors, 4 mice) mice. c IF staining for IDO1 and Lysozyme (LYZ1) in Stat1^flox/flox Apc^Min and Stat1^∆IEC Apc^Min colon tumors. Single fluorescent channels and composites are shown. Positive or double-positive cells are indicated by arrowheads. Scale bars indicate 50 or 10 µm (high-magnification insets). d Quantification of LYZ1⁺ tumor cells of Stat1^flox/flox Apc^Min (si and colon: 12 tumors, 4 mice) and Stat1^∆IEC Apc^Min (si: 8 tumors, colon 12 tumors, 4 mice each) tumors. e, f Quantification of percentages of LYZ1⁺ IDO1⁺ tumor cells between IDO1⁺ (e) and LYZ1⁺ (f) tumor cells of Stat1^flox/flox Apc^Min tumors. g IF staining for IDO1 and MMP7 in Stat1^flox/flox Apc^Min and Stat1^∆IEC Apc^Min colon tumors. Single fluorescent channels and composites are shown. Positive or double-positive cells are indicated by arrowheads. Scale bars indicate 50 µm. h IF staining for IDO1, MMP7, and E-Cadherin in Stat1^flox/flox Apc^Min tumors of the small intestine and colon. Triple-positive cells are indicated by arrowheads. Scale bars indicate 50 µm. i, j Quantification of IDO1⁺ (i) and MMP7⁺ (j) tumor cells in IF-stained tumors of Stat1^flox/flox Apc^Min (si: 7 tumors, 3 mice; colon: 12 tumors, 3 mice) and Stat1^∆IEC Apc^Min (si: 9 tumors, 3 mice; colon: 11 tumors, 3 mice) mice. k, l Quantification of percentages of MMP7⁺ IDO1⁺ tumor cells between IDO1⁺ (k) and MMP7⁺ (l) tumor cells of Stat1^flox/flox Apc^Min tumors. m, n IHC staining (m) and quantification (n) of IDO1⁺ Paneth cells in early and advanced Stat1^flox/flox Apc^Min tumors (si and colon: 12 early adenomas, 5 mice each and 13 advanced tumors, 4 mice each). Scale bars indicate 50 µm. si: small intestine. n.d.: not detectable. Bars represent mean ± SEM.