Figure 7.

Schematic diagram of the in vivo experiments. (A) 72 hours prior to anti-Thy1.1 antibody injection animals were daily treated with sirolimus or phosal (vehicle). At day 4, urine was collected from the first group and these mice were sacrificed. Group 2 received treatments for additional 3 days. At day 7, urine was collected from group 2 and these mice were sacrificed. (B) Transgenic Thy.1.1 mice were injected with the anti-Thy1.1 antibody and urine was collected after 10 days. From day 11 onwards, mice were treated with sirolimus or the vehicle daily. Additional urine was collected at day 17, day 24 and day 31. Mice were sacrificed at day 31.