Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 22;10:8581. doi: 10.1038/s41598-020-65620-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Redox status assessed by means of DCF staining in A549 and Raw 267.4 cells. The redox status, expressed as intensity of DCF fluorescence, was evaluated in the two cell lines, either untreated (Ctr) or exposed for 3 h to varying concentrations either of slag powders with varying granulometry (A1–A5, B1–B5, C1–C5) or of untreated asbestos (D1–D5). H₂O₂ (100 µM) was tested as a positive control. The results are expressed as the average of fluorescence (FU)/number of cells and are the means ± SD of three independent experiments. Statistical analysis: ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 vs. control. (B,C) Representative microphotographs showing the effect of slag powders in Raw 267.4 cell lines, either unstained (B) or loaded with DCFH-DA (C). (D) TBARS levels, expressed as nmol/mg protein, in A549 and Raw 267.4 cells after contact for 12 h with the highest concentrations either of slag powders (15.62 µg/cm²) of different granulometries (A–C) or of asbestos. (D) The columns report the means + SD of triplicate analyses. Statistical analysis: ^bP < 0.01 and ^cP < 0.001 vs. controls.