Table 2.
Viability of A549 cells and Raw 264.7 cells exposed for varying time periods either to asbestos or to SHS-treated slags of different granulometry (see Fig. 2).
| Tested material | Amount (µg/cm2) | Raw 264.7 cells | A549 cells | ||||
|---|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | ||
| Asbestos | 0.33 (D1) | 114.8 ± 2.10 | 116.1 ± 7.98a | 122.7 ± 9.85b | 98.5 ± 2.30 | 119.5 ± 1.30c | 82.6 ± 5.57b |
| 1.00 (D2) | 106.1 ± 1.76 | 104.1 ± 7.60 | 108.0 ± 6.30a | 99.5 ± 3.30 | 115.8 ± 5.60c | 88.4 ± 6.53a | |
| 3.33 (D3) | 87.6 ± 2.21b | 72.6 ± 8.88b | 50.0 ± 4.11c | 97.2 ± 3.10 | 114.7 ± 4.60c | 89.6 ± 8.30a | |
| 7.58 (D4) | 83.9 ± 1.29b | 59.1 ± 8.99c | 45.5 ± 7.09c | 82.5 ± 1.50a | 103.2 ± 2.60 | 88.2 ± 6.59 | |
| 15.62 (D5) | 87.4 ± 1.69b | 70.6 ± 2.60c | 60.3 ± 3.08c | 79.0 ± 0.70a | 57.1 ± 4.60c | 54.9 ± 5.60c | |
| Powder <10 µm | 0.33 (A1) | 96.8 ± 0.60 | 95.8 ± 1.70 | 99.1 ± 7.32 | 113.1 ± 2.10 | 104.5 ± 8.70 | 92.5 ± 1.20b |
| 1.00 (A2) | 98.6 ± 0.90 | 102.7 ± 4.51 | 105.5 ± 8.94 | 119.9 ± 3.60 | 105.2 ± 6.30 | 94.5 ± 8.40b | |
| 3.33 (A3) | 98.6 ± 1.40 | 103.0 ± 6.20 | 116.4 ± 8.81b | 111.3 ± 1.60 | 103.3 ± 5.30 | 87.1 ± 9.40b | |
| 7.58 (A4) | 101.2 ± 0.80 | 103.0 ± 3.40 | 120.9 ± 9.62c | 98.6 ± 2.40 | 81.2 ± 2.90b | 86.5 ± 6.08b | |
| 15.62 (A5) | 100.5 ± 1.80 | 122.2 ± 4.23c | 113.3 ± 6.70b | 93.8 ± 0.90 | 91.5 ± 3.00a | 93.7 ± 6.20 | |
| Powder <3 µm | 0.33 (B1) | 98.1 ± 2.11 | 83.8 ± 4.50 | 118.2 ± 3.20b | 106.2 ± 1.10 | 94.8 ± 4.50 | 77.6 ± 6.80b |
| 1.00 (B2) | 97.8 ± 1.25 | 89.8 ± 6.32 | 124.5 ± 4.00c | 104.2 ± 1.60 | 97.2 ± 4.80 | 70.4 ± 3.70c | |
| 3.33 (B3) | 98.3 ± 2.20 | 94.8 ± 3.84 | 126.4 ± 8.09c | 113.0 ± 2.90a | 92.3 ± 4.10b | 71.3 ± 6.30c | |
| 7.58 (B4) | 91.3 ± 1.28 | 110.5 ± 7.20a | 103.4 ± 6.33 | 113.8 ± 2.20b | 84.1 ± 2.50b | 69.5 ± 7.50c | |
| 15.62 (B5) | 101.4 ± 1.73 | 113.6 ± 2.30b | 117.8 ± 3.91c | 93.8 ± 0.50a | 88.8 ± 1.00b | 80.7 ± 4.50b | |
| Powder <2 µm | 0.33 (C1) | 96.3 ± 0.80 | 116.1 ± 7.00a | 108.0 ± 11.12 | 103.8 ± 2.90a | 114.7 ± 3.80c | 80.2 ± 5.50b |
| 1.00 (C2) | 100.6 ± 1.82 | 122.7 ± 7.81b | 103.0 ± 9.02 | 89.2 ± 2.30b | 111.8 ± 5.10a | 76.9 ± 2.50a | |
| 3.33 (C3) | 99.6 ± 1.00 | 119.8 ± 3.33b | 108.0 ± 9.89 | 90.5 ± 2.50 | 106.9 ± 5.50b | 67.8 ± 12.28b | |
| 7.58 (C4) | 100.4 ± 0.98 | 103.8 ± 6.11 | 112.5 ± 9.50a | 86.5 ± 1.40 | 109.3 ± 8.40a | 65.8 ± 13.10c | |
| 15.62 (C5) | 85.7 ± 1.71a | 97.7 ± 3.34 | 107.3 ± 2.30a | 93.8 ± 0.80 | 64.8 ± 4.80b | 62.6 ± 3.30c | |
The results are means ± SD of 8 replicates and are expressed as % of viability as compared with untreated controls of the corresponding cell lines.
Statistical analysis: aP <0.05, bP <0.01, and cP <0.001, as compared with untreated controls.