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. 2020 May 22;10:8561. doi: 10.1038/s41598-020-65474-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of RNA-Seq and NanoString results for target genes with RT-qPCR results using different combinations of reference genes. All these differential expression results were generated by comparing chicken IEL-NK cells infected with vvIBDV at 3 days post-infection with cells from uninfected chickens. Results from three biological replicates (n = 3). One biological replicate of each sample consisted of IEL-28.4⁺ cells harvested from 8 chickens.