Table 1.

Total IEL cells, percentage of IEL-28.4⁺ cells and total no of IEL-28.4⁺ cells.

	Control	Day 1	Day 3
Total no. of IEL cells/chicken (million)	19.48 ± 2.34	14.29 ± 2.84*	13.95 ± 3.38*
% of IEL-28.4+ cells	33.61 ± 2.23	37.82 ± 3.21*	42.38 ± 2.12*
Total no of IEL-28.4+ cells/chicken (million)	6.43	5.29*	5.86*

Results from three biological replicates (n = 3). One biological replicate of each sample consisted of IEL cells or IEL-28.4⁺ cells harvested from 8 chickens.

Percentage of IEL-28.4⁺ cell population was quantified by flow cytometry while total number of IEL cells was counted post-harvesting of IEL cells. * indicates significant between different samples using one-way ANOVA (p < 0.05). The value was the means ± SEM of three experiments.