Table 2.
Average count and CV% from NanoString for 15 candidate reference genes.
| Gene | Average count | CV% |
|---|---|---|
| ACTB | 96,218 | 31.04 |
| ALB | 19 | 36.63 |
| B2M | 15,986 | 65.91 |
| G6PDH | 688 | 42.00 |
| GAPDH | 16,943 | 38.22 |
| HMBS | 1,444 | 35.80 |
| HPRT1 | 715 | 34.67 |
| IFNA3 | 28 | 52.21 |
| RPL4 | 15,044 | 44.96 |
| RPL30 | 13,605 | 48.41 |
| SDHA | 4,325 | 31.65 |
| TBP | 350 | 41.40 |
| TUBB1 | 1,998 | 38.27 |
| UB | 70,600 | 40.55 |
| YWHAZ | 4,085 | 27.68 |
Results from three biological replicates (n = 3). One biological replicate of each sample consisted of IEL-28.4+ cells harvested from 8 chickens.